Chapter 9: Microbial Genetics



1.  

What similarities are there in bacterial genetic exchange mediated by transformation, transduction, and conjugation?



2.  

Contrast transformation, transduction, and conjugation with respect to (a) the maximum number of genes transferred, and (b) the proteins required to effect gene transfer.



3.  

Compare the base-pairing requirements and proteins involved in general and site-specific recombination.



4.  

What steps involving the breakage of the sugar-phosphate backbone of DNA are a part of general recombination? How are these breaks repaired?



5.  

What characteristics do cells competent for transformation have? How can these properties be enhanced?



6.  

What characteristics of a phage's life cycle would make it a candidate for a specialized transducing phage, or a generalized transducing phage?



7.  

What type of genes are carried on all plasmids? What genes are required to make a plasmid conjugative?



8.  

By what molecular mechanism are Hfr strains formed?



9.  

Describe the molecular events involved in the transposition of an element like Tn5.



10.  

What similarities and differences are there between meiosis and mitosis?



11.  

At what stages of its life cycle is a yeast cell haploid? diploid?



12.  

Will all point mutations in a gene lead to an observable change in the protein for which it codes? Consider (a) the degeneracy of the genetic code, (b) the chemical similarity of some amino acids to one another (for example, leucine and isoleucine), and (c) the location of the alteration in relation to the active site of the enzyme.



13.  

What effect is the deletion or insertion of one base in a gene likely to have upon the amino acid sequence of the specified protein?



14.  

What similarities and differences are there between insertion sequences and transposons?


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